anti cav3 2 (Alomone Labs)
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anti cav3 2
Anti Cav3 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cav3+2/Anti-CaV3%2E2+(CACNA1H)+Antibody/bio_rxiv__64898__2026__03__19__712770-239-26-29
Average 95 stars, based on 80 article reviews
Anti Cav3 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cav3+2/Anti-CaV3%2E2+(CACNA1H)+Antibody/bio_rxiv__64898__2026__03__19__712770-239-26-29
Average 95 stars, based on 80 article reviews
anti cav3 2 - by Bioz Stars,
2026-11
95/100 stars
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Blocking Assay:Article Title: T-type Ca 2+ channels play a dual role in modulating the excitability of dorsal root ganglia neurons Article Snippet: After protein concentrations were determined with BCA assays, the protein samples were separated on a 10% SDS-PAGE gels and transferred to polyvinylidene-fluoride (PVDF) membranes (Merck Millipore). .. After blocking with 3% fat-free dried milk in TBST (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) at room temperature for 1 h, PVDF membranes were incubated with Article Title: Histone methylation-mediated microRNA-32-5p down-regulation in sensory neurons regulates pain behaviors via targeting Cav3.2 channels Article Snippet: .. After hybridization, the sections were rinsed in washing buffer (containing formamide and saline-sodium citrate) three times and blocked for 1 h in blocking buffer at 37 °C, followed by incubation in Cy3-labeled anti-digoxin antibody (diluted in blocking buffer at 1:150) for 1 h. For co-labeling experiments, the sections were first hybridized with the probe of miRNA-32-5p, then co-incubated with rabbit Incubation:Article Title: T-type Ca 2+ channels play a dual role in modulating the excitability of dorsal root ganglia neurons Article Snippet: After protein concentrations were determined with BCA assays, the protein samples were separated on a 10% SDS-PAGE gels and transferred to polyvinylidene-fluoride (PVDF) membranes (Merck Millipore). .. After blocking with 3% fat-free dried milk in TBST (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) at room temperature for 1 h, PVDF membranes were incubated with Article Title: Cav3.2 T-Type calcium channels downregulation attenuates bone cancer pain induced by inhibiting IGF-1/HIF-1α signaling pathway in the rat spinal cord Article Snippet: According to a standard protocol, an equal amount (40 μg) of protein was separated from each sample on 8% – 12% SDS-PAGE and transferred to the polyvinylidene fluoride membrane (Merck Millipore). .. The blots were blocked in a solution of 5% non-fat milk with TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, 0.05% Tween 20) for 1 h at room temperature and incubated with one of the following primary antibodies at 4 °C overnight: rabbit Article Title: Histone methylation-mediated microRNA-32-5p down-regulation in sensory neurons regulates pain behaviors via targeting Cav3.2 channels Article Snippet: .. After hybridization, the sections were rinsed in washing buffer (containing formamide and saline-sodium citrate) three times and blocked for 1 h in blocking buffer at 37 °C, followed by incubation in Cy3-labeled anti-digoxin antibody (diluted in blocking buffer at 1:150) for 1 h. For co-labeling experiments, the sections were first hybridized with the probe of miRNA-32-5p, then co-incubated with rabbit Cell-Signaling:Article Title: Cav3.2 T-Type calcium channels downregulation attenuates bone cancer pain induced by inhibiting IGF-1/HIF-1α signaling pathway in the rat spinal cord Article Snippet: According to a standard protocol, an equal amount (40 μg) of protein was separated from each sample on 8% – 12% SDS-PAGE and transferred to the polyvinylidene fluoride membrane (Merck Millipore). .. The blots were blocked in a solution of 5% non-fat milk with TBST (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, 0.05% Tween 20) for 1 h at room temperature and incubated with one of the following primary antibodies at 4 °C overnight: rabbit Article Title: Histone methylation-mediated microRNA-32-5p down-regulation in sensory neurons regulates pain behaviors via targeting Cav3.2 channels Article Snippet: .. After hybridization, the sections were rinsed in washing buffer (containing formamide and saline-sodium citrate) three times and blocked for 1 h in blocking buffer at 37 °C, followed by incubation in Cy3-labeled anti-digoxin antibody (diluted in blocking buffer at 1:150) for 1 h. For co-labeling experiments, the sections were first hybridized with the probe of miRNA-32-5p, then co-incubated with rabbit Hybridization:Article Title: Histone methylation-mediated microRNA-32-5p down-regulation in sensory neurons regulates pain behaviors via targeting Cav3.2 channels Article Snippet: .. After hybridization, the sections were rinsed in washing buffer (containing formamide and saline-sodium citrate) three times and blocked for 1 h in blocking buffer at 37 °C, followed by incubation in Cy3-labeled anti-digoxin antibody (diluted in blocking buffer at 1:150) for 1 h. For co-labeling experiments, the sections were first hybridized with the probe of miRNA-32-5p, then co-incubated with rabbit Saline:Article Title: Histone methylation-mediated microRNA-32-5p down-regulation in sensory neurons regulates pain behaviors via targeting Cav3.2 channels Article Snippet: .. After hybridization, the sections were rinsed in washing buffer (containing formamide and saline-sodium citrate) three times and blocked for 1 h in blocking buffer at 37 °C, followed by incubation in Cy3-labeled anti-digoxin antibody (diluted in blocking buffer at 1:150) for 1 h. For co-labeling experiments, the sections were first hybridized with the probe of miRNA-32-5p, then co-incubated with rabbit other:Article Title: Effects of progesterone on T-type-Ca 2+ -channel expression in Purkinje cells. Article Snippet: Immunostaining of 12-μm-thick cryosections was performed on SuperFrost Plus Adhesion slides (Thermo Fisher Scientific, Waltham, MA, USA, J1800AMNZ). Article Title: Effects of progesterone on T-type-Ca 2+ -channel expression in Purkinje cells Article Snippet: The next day, after thorough washing with PBS (2 × 2 minutes plus 2 × 10 minutes), the samples were incubated with goat anti-mouse tetramethylrhodamine (TRITC) antibody (1:1000 in PBS T5393, MilliporeSigma; RRID: AB_261699) at room temperature for 2 hours. |
